Fig. 2: Valence-related activity in aIC and pIC glutamatergic neurons.

a Schematic of sucrose/quinine consumption test. b Peri-sucrose licking time course of calcium signal in the aIC (n = 13 mice) and pIC (n = 9 mice). c Calcium signal increases during sucrose licking in aIC (n = 13 mice) and pIC (n = 9 mice) glutamatergic neurons compared to baseline (Two-way ANOVA, F_(1,20) = 19.57, ***p = 0.0003 for the event (baseline, licking) with no effect of the region (aIC, pIC) and no interaction; Bonferroni test for aIC *p = 0.017, and for pIC **p = 0.007). d Peri-event analysis of the calcium signal between baseline and movement onset toward the sucrose port in the aIC and pIC (aIC n = 13 mice, pIC n = 9 mice). e Calcium signal increased after movement towards the sucrose port in aIC neurons compared to baseline without changes for pIC neurons (n = 13 and n = 9 mice, Two-way ANOVA, F_(1,20) = 8.802, **p = 0.008 for the event (baseline, move to port) with no effect of the region (aIC, pIC) and no interaction; Bonferroni test for aIC **p = 0.007). f Peri-quinine licking time course of calcium signal in the aIC (n = 10 mice) and pIC (n = 8 mice). g Calcium signal increases during quinine licking in pIC neurons (n = 8 mice), but not aIC neurons (n = 10 mice) compared to baseline (Two-way ANOVA, F_(1,16) = 6.957, *p = 0.018 for the event (baseline, licking), with no effect of region and no interaction; Bonferroni test for pIC *p = 0.019). h Schematic of the tail suspension test. i Peri-event calcium signal in the aIC (n = 10 mice) and pIC (n = 10 mice) before and during tail suspension. j Calcium signal increases post-lift in aIC (n = 10 mice), but not pIC (n = 10 mice) neurons, compared to pre-lift (Two-way ANOVA, F_(1,18) = 49.54, ***p < 0.001 for the event (pre- vs post-lift), no effect of the region (aIC, pIC), and F_(1,18) = 21.04, ***p = 0.0002 for event x region interaction; Bonferroni test for aIC, ***p < 0.0001) and is higher in aIC compared to pIC post-lift (Bonferroni test **p = 0.003). k Schematic of the footshocks test. l Peri-shock time course of calcium signal in the aIC (n = 10 mice) and pIC (n = 11 mice). m Calcium signal increases post-shock in aIC (n = 10 mice), but not pIC (n = 11mice) neurons compared to pre-shock (Two-way ANOVA, F_(1,19) = 17.84, ***p = 0.005 for the event (pre- vs post-shock) with no effect of the region (aIC, pIC) and no interaction; Bonferroni test for aIC **p = 0.002), and is higher in aIC compared to pIC post-shock (Bonferroni test *p = 0.032). n Post-shock calcium signal in aIC neurons negatively correlates with the time mice spent in open arms of the EPM (One-tailed Pearson correlation: R² = 0.57, **p = 0.006, n = 10 mice). o Post-shock calcium signal in pIC neurons positively correlates with the time mice spent in open arms of the EPM (One-tailed Pearson correlation: R² = 0.29, *p = 0.043, n = 11 mice). All the results are represented as mean ± SEM.