Fig. 6: Role of aIC-BLA neurons in valence-related behaviors.

a Two representative traces showing an occupancy heatmap of the time spent in the non-stimulated (left) or stimulated (right) side for a control eYFP mouse (left) and GtACR2 mouse (right). b Preference index is increased in GtACR2 group where aIC-BLA glutamatergic neurons are inhibited compared to fRed control group (Two-tailed unpaired t-test, t = 2.345, *p = 0.035, fRed n = 8 mice, GtACR2 n = 7 mice). c Schematic of sucrose or quinine consumption test. d Peri-licking analysis of the calcium signal between sucrose (blue) or quinine (orange) pre-lick and after licking onset. e Global calcium signal after sucrose licking onset is decreased compared to pre-lick (Two-tailed paired t-test, t = 4.077, **p = 0.001, n = 15 mice). f Bar plot of global calcium signal during quinine pre-lick and after licking onset (n = 15 mice). g Peri-event analysis of the calcium signal between pre-suspension and suspension. h Global calcium signal is increased during the suspension compared to pre-tail suspension (Two-tailed paired t-test, t = 6.196, ***p = 0.0003, n = 9 mice). i The intensity of calcium signal during tail suspension is negatively correlated with the time mice spent in the open arms of the EPM (One-tailed Pearson correlation: R² = 0.498, *p = 0.02, n = 9 mice). j Peri-event analysis of the calcium signal between pre- and post-shock (n = 18 mice). k Global calcium signal is increased during post-shock compared to pre-shock (Two-tailed paired t-test, t = 3.281, **p = 0.0044, n = 18 mice). l Global signal post-shock correlates positively with global signal in the open arms of the EPM (One-tailed Pearson correlation: R² = 0.2846, *p = 0.01, n = 18 mice). All the results are represented as mean ± SEM.