Fig. 2: The role of β3 integrins in inhibiting thrombin-induced RhoA activation. | Nature Communications

Fig. 2: The role of β3 integrins in inhibiting thrombin-induced RhoA activation.

From: Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis

Fig. 2

a, b Rhotekin-RBD bead pull down assay was used to analyze RhoA activation in WT or β3-/- mouse platelets stimulated with thrombin (0.03 U/mL) at various time points, 4 independent experiments. c, d RhoA pull down assay with RGDS or RGES-pre-treated (2 mM, 3 min, 22 °C) mouse platelets stimulated with thrombin (0.03 U/mL) for the indicated time points, four independent experiments. e, f RhoA pull down assay with 0.03 U/ mL thrombin-stimulated mouse platelets pre-treated with control peptide or mP6 peptide (100 μM, DMSO) for 3 min at 22 °C, three independent experiments. a, c, e Representative western blots; b, d, f quantification of western blot images as analyzed using ImageJ. All data are shown as mean ± SEM. Statistical significance was determined using Student’s t test, (f) paired Student’s t test, two tailed. Source data are provided as a Source Data file.

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