Fig. 2: FFN270 labels NE axons in the cerebellum.
From: Norepinephrine release in the cerebellum contributes to aversive learning
a Schematic of cerebellar brain slice preparation and incubation with FFN270 with or without nomifensine (created with BioRender.com). b, c Representative 2-photon microscopy images of FFN270 (10āĪ¼M) loaded into the NE axons of acute cerebellar slices (lobule IV; one representative image of 4 independent experiments is shown in b and c). d The FFN270 axonal labeling was inhibited by nomifensine (lobule IV; NOM, 5āĪ¼M; representative of 3 independent experiments). PCL Purkinje cell layer, GL granular layer, and ML molecular layer. The scale bar is 5 Ī¼m in (b); 100 Ī¼m in (c and d).
