Fig. 4: Fiber reduces signatures of high redox metabolic activity and reduces abundance of complex 1 bacteria. | Nature Communications

Fig. 4: Fiber reduces signatures of high redox metabolic activity and reduces abundance of complex 1 bacteria.

From: Fiber supplementation protects from antibiotic-induced gut microbiome dysbiosis by modulating gut redox potential

Fig. 4

(a) Significant differences in HUMaN3 reaction expression during D5 of experiment across the redox tower as determined by MaAsLin2. Coefficient shown on x-axis and size of dot represents q-val = FDR. Full results in Supplementary Information. (b) Heat map depicting abundance of MAGs containing complex 1 D1 and D5 (c) during experiment. White boxes represent values outside of the scale. Change in abundance of complex 1 MAGs shown with two-tailed Mann–Whitney for significance D1, glucose p value = 0.0043, fiber p value = 0.0087 (d) and D5, glucose p value = 0.0022, fiber p value = 0.0260 (e) of experiment (n = 6) *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (f) Changes in expression of superoxide dismutase, adj p values left to right = 0.0009, 0.0023, 0.0374, 0.0005. Changes in expression of NAD(P)H dehydrogenase (quinone), adj p values left to right = 0.0033, 0.0119, 0.0268, 0.0006. (g) Changes in expression of Nitrate reductase (cytochrome), adj p values left to right = 0.0478, <0.0001, 0.0478, 0.0240, 0.0449, 0.0081, 0.0161. For f, g: (n = 6) Copm = copies per million reads. Mean ± SEM Kruskal Wallis with Dunn’s Correction *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (h) eH and pH values from additional mouse experiment (n = 6). Mean ± SEM Significance determined by two-tailed Mann–Whitney test, p values left to right = 0.0080, 0.0078, 0.0080. (i) Pourbaix diagram depicting eH and pH values from lyophilized cecal contents of mice with and without antibiotics measured within 24 h after rehydration with RO water. (j) Conclusion schematic.

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