Fig. 1: Esg>yki^[S3A] gut tumors induce alterations in energy metabolism and myofiber degradation.

a Immunostaining of a Lpp-GAL4 hemi-thorax (wildtype) expressing UAS-TransTimer⁷⁷ (Lpp>Transtimer, GFP) for 4 days to label fat body cells (green) actively expressing Lpp. Actin myofibers were stained with phalloidin (magenta). Trachea is observed in the DAPI channel. b, c Triglyceride (b) and glucose (c) content in thoraces (Triglycerides: day 8 and day 14 p < 0.0001****. Glucose: day 8 and day 14 p < 0.0001****). N = 12 biologically independent samples, N = 8 thoraces per sample. Triglycerides and glucose were calculated from the same thoraces. Results were reproduced in three independent experiments (b, c). d–g Immunostaining of the flight muscles of hemi-thoraces at different magnifications (20× d, e; 60× f, g). Myofibrils were labelled with phalloidin (magenta) and mitochondria with CoxIV (green). h–j Percentage of hemi-thoraces showing myofiber degradation as shown in (e, g) (p < 0.0001****). Samples were analyzed at 14 or 20 days (h) or 19 days (i, j) after tumor induction. Total number of hemi-thoraces scored per genotype is shown. Data shows mean with ±SD (b, c). Values were normalized to the mean of control samples of 2 days after tumor induction (b, c). Statistical analysis was done using two-way ANOVA with Sidak correction test for multiple comparisons (b, c), or two-tailed Fisher’s exact test with a confidence interval of 95% for pairwise comparisons between two groups (h–j). Scale bar is 100 µm in (a, d, e) and 5 µm in (f, g). Source data are provided as a Source Data file.