Fig. 2: REPTOR regulates glucose metabolism in Esg>yki[S3A] thoraces.
From: REPTOR and CREBRF encode key regulators of muscle energy metabolism
a mRNA levels of REPTOR in thoraces (8 days p = 0.004**, 14 days p < 0.0001****, 20 days p = 0.0025**). N = 4 biologically independent samples, N = 6–10 thoraces per sample. b, c Protein levels of phosphorylated S6K (pS6K) and total S6K (b), or REPTOR (c) measured from thoraces after 14 days of tumor induction. N = 2 biologically independent samples (b, c). Results were reproduced in two (b) or three (c) independent experiments. Values indicate densitometry of bands normalized to control. d mRNA levels of REPTOR in thoraces (Ctrl vs yki[S3A] p < 0.0001****, yki[S3A] vs yki[S3A] REPTOR RNAi p < 0.0001****). N = 6 biologically independent samples, N = 6–10 thoraces per sample. e, f Glucose content in thoraces upon REPTOR knockdown in muscle (e: Ctrl vs yki[S3A] p < 0.0001****, yki[S3A] vs yki[S3A] REPTOR RNAi p = 0.0033**. f: p = 0.93 ns). N = 12 biologically independent samples, N = 8 thoraces per sample. Results were reproduced in three independent experiments. g Scheme of the glycolytic pathway and mRNA levels of Phosphofructokinase (pfk) in thoraces (Ctrl vs yki[S3A] p = 0.0003***, yki[S3A] vs yki[S3A] REPTOR RNAi p = 0.0076**). N = 6 biologically independent samples, N = 6–10 thoraces per sample. Samples were analyzed after 12 days of gene induction (d–g). Data shows mean with ±SD (e, f) or boxplots (median and quartiles) with whiskers (minimum to maximum) (a, d, g). Values were normalized to the mean of control samples of 2 days after tumor induction (a) or mean of control samples (d–g). Statistical analysis was done using two-way ANOVA (a), one-way ANOVA with Sidak correction test for multiple comparisons (d, e, g), or two-tailed t-test with Welch’s correction (f). Source data are provided as a Source Data file.
