Fig. 1: Differential expression of cellular protein signatures during peripheral B cell maturation.

a Representative flow cytometry plots identifying T1 (CD19⁺ CD93⁺ IgM⁺ CD23^-), T2 (CD19⁺ CD93⁺ IgM⁺ CD23⁺), MZ (CD19⁺ CD93^- CD21⁺ CD23^-) and FoB (CD19⁺ CD93^- CD21^- CD23⁺) cells. Full gating strategy is reported in Supplementary Fig 9a. b Proportion of proteins that were unchanged or differentially expressed within T1, T2, MZ and FoB cells based on ANOVA test followed by a Benjamini-Hochberg multiple testing correction with FDR < 0.05. Numbers indicate proteins that were identified in at least three out of four biological replicates and by more than one unique peptide. The full list of proteins is provided in Supplementary Data 1a. c Heat map showing the log2-fold deviation from the mean of normalized copy numbers of 1816 DE proteins as in b. d Venn diagram indicates number of proteins that were found in one or more B cell subsets based on their copy numbers. Example of proteins that were exclusively found in T1 or MZ cells are reported on the left of the diagram. All proteins are listed in Supplementary Data 1b, c. In b–d, B cells were sorted from n = 4 biologically independent replicates from two independent experiments. Source data of (c) are provided as Source Data file.