Fig. 8: Genetic depletion of Egr1 attenuated fibrotic remodelling in mice with conditional myofibroblast-specific Irx2 overexpression after angiotensin II (Ang II) infusion. | Nature Communications

Fig. 8: Genetic depletion of Egr1 attenuated fibrotic remodelling in mice with conditional myofibroblast-specific Irx2 overexpression after angiotensin II (Ang II) infusion.

From: IRX2 regulates angiotensin II-induced cardiac fibrosis by transcriptionally activating EGR1 in male mice

Fig. 8

Conditional myofibroblast-specific Irx2-overexpressing mice (Irx2 mfTg) were bred with Egr1 global knockout mice or wild type (WT). The resulting mouse line and littermate controls were subjected to Ang II infusion for 4 weeks. A Heart weight-to-tibia length (HW/TL) ratio (n = 10 mice, Irx2 Tgfl/fl + WT+Ang II; n = 10 mice, Irx2 Tgfl/fl+Egr1 KO+Ang II; n = 11 mice, Irx2 mfTg+WT+Ang II; n = 11 mice, Irx2 mfTg+Egr1 KO+Ang II). B Histological staining showed cardiomyocyte enlargement and cardiac fibrosis after Ang II infusion (n = 5). C Cardiac fibrosis was determined by picrosirius red staining after Ang II infusion (n = 5). D The cell area of cardiomyocytes was determined with wheat germ agglutinin (WGA) staining after Ang II infusion (n = 5). E, F The ejection fraction (EF) (E) and left ventricular end-diastolic dimension (LVIDd) (F) were detected in these groups (n = 6). Data are shown as the mean ± SEM, and analyzed using one-way ANOVA followed by Tukey post hoc test (A, C, and D). For the analysis in (E, F), repeated measures ANOVA was conducted. Source data are provided as a Source Data file.

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