Fig. 3: Migration dynamic of RBI-derived and CHT-derived neutrophils during inflammation.
From: Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish
A Representative images of the tail at 3 h post damage (hpd) showing RBI-derived and CHT-derived neutrophil tracks to reach the wound. The blue line represents the circulatory loop. B Quantification of the migration route (bloodstream or extracellular matrix) utilized by RBI-derived and CHT-derived neutrophils to reach the wound at 1, 2, and 3 hpd. Analysis of the (C) Euclidian distance, (D) maximal velocity, (E) cumulative distance and (F) directionally of RBI-derived and CHT-derived neutrophils recruited to the wound. n = 13 larvae per experiment Statistical analyses were done with a two-sided Mann-Whitney U test. Data are represented as mean ± SD (G) Dispersion. Rose diagram showing the deviation angles in each segment of the route followed by neutrophils with respect to the direct route (0°) to the damage area. White numbers inside the green or red bars represent the percentage of neutrophils that follow a segment with this angle. The p-values were obtained after applying a Rayleigh test to angles distribution in each subpopulation. n = 10 larvae per experiment. Experiments were performed independently at least 3 times.
