Fig. 7: Csf3b regulates RBI-derived neutrophil egress from hematopoietic tissue at steady state.

A Experimental strategy. At 34 hpf the yolk or CHT of control and morphant embryos was photoconverted and at 44 hpf the amount of RBI-derived and CHT-derived neutrophils was quantified. B Quantification of RBI-derived and CHT-derived neutrophils in and outside the corresponding hematopoietic region in control and morphant embryos. n = 13 embryos for yolk or CHT photoconvertion per experiment. C Experimental strategy. At 34 hpf and 44 hpf the head of control and morphant embryos was photoconverted, at 54 hpf caudal fin transection was performed and the number of RBI-derived and CHT-derived neutrophils present at the wound was quantified during the first three hours post damage. n = 18 larvae per condition per experiment. D Quantification of the number of RBI-derived and CHT-derived neutrophils present at the wound at 0, 1, 2 and 3 hpd in control and morphant embryos. Statistical analysis was done using a two-sided Kruskal-Wallis Test. Data are represented as mean ± SD Experiments were performed independently at least 3 times.