Fig. 5: Model CTCs for molecular profiling including RNA sequencing and Gene Ontology (GO) enrichment.
From: Zwitterionic microgel preservation platform for circulating tumor cells in whole blood specimen
a The RNA integrity number (RIN) values of model CTCs isolated from fresh or preserved blood specimens. b Scaled heatmap of specific breast cancer gene expression in the model CTCs from the fresh or preserved blood specimens. c Principal component analysis (PCA) of CTCs from the fresh or preserved blood specimens. Significant gene expression changes in model CTCs from the ZBVA hydrogel preservation system (d) and CPDA solution (e). f Top ten significant changes in biological processes (BP), cellular component (CC), and molecular function (MF) in CTCs preserved in the ZBVA hydrogel on the 7th day. The y axis on the left indicates the significance score shown as -log(padj). The right y axis indicates the ratio of the number of GO pathway correlated genes that map the total significant expressed genes (green line). The pink dashed line indicates where the -log(padj) equals 1.3, which is the boundary score between significance and non-significance. For a, values represent mean ± s.d. of n = 3 independent experiments and the two-tailed Student’s t test related p values are indicated.
