Fig. 1: Protected vaccine recipients show coordinated humoral immune response.
From: Antibody-mediated NK cell activation as a correlate of immunity against influenza infection
Serum samples, day 28 post vaccination, were analyzed for HAI, influenza-specific antibody levels, and influenza-specific antibody-mediated innate immune functionality. A shows H3N2 A/Victoria/361/2011 HAI titers for controls (n = 86 individuals, yellow) who remained uninfected and cases (n = 14 individuals, blue) who became infected during the season. B shows H3-specific IgG1 levels by Luminex (median fluorescence intensity, MFI) for controls (n = 86) and cases (n = 14), wherein each dot represents the mean of two technical replicates for a single individual. A, B lines show median values and differences are not significant by two-sided Mann–Whitney U test. C Heat map depicts responses across all measured antibody features for cases and controls. Each row represents an individual sample. Each column represents a measured feature for the listed antigen. Breadth features reflect antigen-specific antibody isotype and FcR binding across all tested strains (Table S3). Values for all measurements were Z score normalized with Z score values depicted on the color map. Rows were manually clustered by infection status. ADCP antibody-dependent cellular phagocytosis, ADNP antibody-dependent neutrophil phagocytosis, ADCD antibody-dependent complement deposition, ADNKA antibody-dependent NK cell activation, ADDCP antibody-dependent Dendritic Cell phagocytosis. D Correlation matrices show Spearman R correlations between H3-specific antibody-dependent functions for controls and cases. The size of the circles and the color of the circles represent the strength of the correlation, with red for positive and blue for negative correlations. Source data are provided as a Source Data file.
