Fig. 4: High-dose vaccination enhances HAI but not antibody-mediated NK cell activation.
From: Antibody-mediated NK cell activation as a correlate of immunity against influenza infection
A shows H3N2 A/Victoria/361/2011 HAI titers for regular-dose vaccinees and high-dose vaccinees. B shows H3-specific IgG1 levels by Luminex (median fluorescence intensity, MFI) for RD (purple) and HD (green) recipients. A, B Each dot represents the mean of two technical replicates for a single individual. Bars represent medians and significance tested by two-sided Mann–Whitney U test, *p < 0.05. C Heat map depicts responses across all measured antibody features for RD and HD recipients. Each row represents an individual sample. Each column represents a measured feature. Values for all measurements were z score normalized. D shows H3-specific FCGR3A-binding levels for regular and high-dose vaccinees. E shows H3-specific NK cell CD107a expression for regular and high-dose vaccinees. F shows H3-specific NK cell MIP-1b expression for regular and high-dose vaccinees. G shows percentage of H3-specific antibodies that have fucosylated Fc glycans. D–G Each dot represents the mean of two technical replicates for a single individual. Bars represent medians and significance tested by Mann–Whitney U test, not significant. A–G regular dose vaccinees n = 50; high-dose vaccinees n = 50 individuals. See also Figure S5. Source data are provided as a Source Data file.
