Fig. 4: Demonstration of prostate cancer detection in a preparation of fresh frozen tissue sections.
From: Infrared spectroscopic laser scanning confocal microscopy for whole-slide chemical imaging
a Rough annotations guided by pathologists are (b) transferred from H&E images to IR images on consecutive sections. c A deep learning model is trained to predict epithelial (epi.) malignancy using multispectral IR images. d Validation is performed on unstained samples containing benign glands, malignant glands, and benign glands surrounded by malignant tumors, each imaged with IR-LSM and represented in each row. e Target ROIs are shown on the Amide I map, representing a full microscopy slide, with (f) indicative IR-LSM 10× magnified field of view simulating real-time screening. g, h Comparative images of H&E-stained adjacent sections of the same tissue sample are shown with corresponding ROIs marked.
