Fig. 4: The intratumoral T cell phenotypes are altered upon resistance development. | Nature Communications

Fig. 4: The intratumoral T cell phenotypes are altered upon resistance development.

From: Acquired resistance to anti-PD1 therapy in patients with NSCLC associates with immunosuppressive T cell phenotype

Fig. 4

a Heat map of T cell marker expression (y-axis) and phenotypic clusters (x-axis) were calculated from IMC raw data. Marker expressions were calculated using the mean intensities for each marker, counts were arcsinh transformed and the clusters were defined using PhenoGraph. The top color bar defines different T cell subsets present in the tumors. b Cluster compositions of tumor samples taken from each patient initially and upon resistance development. c Percentages of each T cell subtype in tumor samples taken from each patient initially and upon resistance development. d t-distributed stochastic neighbor embedding (t-SNE) map of all T cells color-coded by each patient. e t-SNE plot overall T cells color-coded by the different T cell subtype defined in the heat map. f t-SNE plot color-coded by responding and resistant tumors.

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