Fig. 5: Changes in the myeloid compartment due to resistance detected by IMC differ in each patient.

a Heat map of myeloid cell marker expression (y-axis) and phenotypic clusters (x-axis) were calculated from IMC raw data. Marker expressions were calculated using the mean intensities for each marker, counts were arcsinh transformed and the clusters were defined using PhenoGraph. The top color bar defines different myeloid cell subsets present in the tumors. b Percentages of cells from each patient in indicated clusters at response and at resistance. c Percentages of macrophages in M1 (defined as CD169⁺, HLA-DR⁺, STING⁺, and CD38⁺ myeloid cells) and M2 states (define as CD163⁺, CD204⁺, and CD206⁺ myeloid cells) at response and resistance for each patient. d t-SNE plot of all macrophages color-coded by patient origin. e t-SNE plot color-coded by M1 and M2 macrophage types. f t-SNE plot of macrophages color-coded by responding and resistant tumors.