Fig. 3: Interactive FCM data analysis with CRUSTY. | Nature Communications

Fig. 3: Interactive FCM data analysis with CRUSTY.

From: CRUSTY: a versatile web platform for the rapid analysis and visualization of high-dimensional flow cytometry data

Fig. 3

a Definition of the MAIT signature according to GZMK, CD161, and CD127 expression, and enrichment of the signature on the UMAP plot after clicking the “drop” function (hand cursor). The signature is arbitrarily divided in low, medium (med) and high. b MAIT signature levels across VIA clusters and tissue specimens in the dataset. c Isolation of MAIT signaturehigh cells from (a). d Color-coded expression of CD69 on MAIT signaturehigh cells from c (top) and on a standard CD69 vs. CD45RO bivariate plot (bottom). In the latter, channel values were rescaled from 0 to 1. PBMC peripheral blood mononuclear cells; NSCLC non-small cell lung cancer; MAIT mucosal-associated invariant T cell.

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