Fig. 2: Morphological characterization of rmf1 rmf2.
From: SCFRMF mediates degradation of the meiosis-specific recombinase DMC1
a Whole plant phenotypes of Col-0, rmf1-1, rmf2-1, and rmf1-1 rmf2-1. rmf1-1 and rmf2-1 plants do not show any vegetative growth or fertility abnormalities, while rmf1-1 rmf2-1 has normal vegetative growth but is completely sterile. The white arrows point to siliques in rmf1-1 rmf2-1. Bar = 3 cm. b Siliques of Col-0 rmf1-1, rmf2-1, and rmf1-1 rmf2-1. The rmf1-1 and rmf2-1 plants have siliques similar in length to Col-0, while rmf1-1 rmf2-1 is completely sterile. The white arrows point to siliques in rmf1-1 rmf2-1. Bar = 3 cm. c–f Pollen grains stained with Alexander Red from c Col-0, d rmf1-1, e rmf2-1, and f rmf1-1 rmf2-1. Pollen viability in rmf1-1 and rmf2-1 are similar to Col-0, while dramatically decreased in rmf1-1 rmf2-1. Bar = 100 μm. g–j Tetrad-stage microspores stained with toluidine blue from g Col-0, h rmf1-1, i rmf2-1, and j rmf1-1 rmf2-1. rmf1-1 and rmf2-1 has normal tetrads similar to Col-0, while rmf1-1 rmf2-1 has polyads. Bar = 5 μm. k Scatter plot of viable pollen number. Pollen viability in rmf1-1 and rmf2-1 have no significant difference compared with Col-0, while significantly decreased in rmf1-1 rmf2-1. Data are presented as the mean values ± SD, p values were calculated using a two-tailed Student’s t-test. For pollen grain viability analysis, 22 anthers for Col-0, 23 anthers for rmf1-1, 24 anthers for rmf2-1, 26 anthers for rmf1-1 rmf2-1 isolated from more than three independent plants were used. l Histogram of the proportion of normal versus abnormal tetrads. Abnormal tetrads were observed in rmf1-1 rmf2-1. Data are presented as the mean values ± SD, p values were calculated using a two-tailed Student’s t-test. m Meiotic chromosome phenotypes of Col-0 and rmf1-1 rmf2-1 assayed by centromere FISH. rmf1-1 rmf2-1 is defective in typical pachytene chromosome and bivalent formation. Bar = 5 μm. For each meiotic stage in Col-0 and rmf1-1 rmf2-1, cells isolated from more than three independent plants were observed with similar meiotic chromosome phenotypes. The number of cells observed was labeled in the figures. Source data are provided as a Source data file.
