Fig. 3: Kinetics of the formation and decay of Co(I) and Co(II) states using stopped flow.
From: Redox driven B12-ligand switch drives CarH photoresponse
a Kinetic transient at 388 nm showing rate of formation of Co(I) for anaerobic TtCBD sample after photoexcitation with a laser pulse at 530 nm (15 mJ). b Kinetic transient at 388 nm showing rate of decay of Co(I) species upon rapid mixing of anaerobically photolysed TtCBD sample with oxygenated buffer from stopped-flow experiments. The inset shows the corresponding absorbance spectra over a range of timepoints. c Kinetic transient at 475 nm showing rate of decay of Co(II) species upon rapid mixing of anaerobically photolysed TtCBD sample with oxygenated buffer from stopped-flow experiments. The inset shows the corresponding absorbance spectra over a range of timepoints. d Kinetic transient at 359 nm showing formation of final bis-His ligated light state over several seconds upon rapid mixing of anaerobically photolysed TtCBD sample with oxygenated buffer from stopped-flow experiments. The inset shows the corresponding absorbance spectra over a range of timepoints. e Scheme describing the changes in Co redox state following light activation under distinct [O2] conditions.
