Fig. 6: Ablation of FAP+-CAFs by FAP-CAR T cells enhances the efficacy of subsequent treatment with TAA Meso-CAR T cells in PDAC tumors. | Nature Communications

Fig. 6: Ablation of FAP+-CAFs by FAP-CAR T cells enhances the efficacy of subsequent treatment with TAA Meso-CAR T cells in PDAC tumors.

From: Desmoplastic stroma restricts T cell extravasation and mediates immune exclusion and immunosuppression in solid tumors

Fig. 6

a Treatment protocol: C57BL/6 mice bearing established 4662 tumors were treated with the indicated first dose of T cells and 15 days later treated with the second dose of indicated T cells. b Average growth curves, c tumor weights at end-point and d modified Kaplan–Meier curves of mice from each of the indicated treatment groups. e Treatment protocol in established KPC mice. f Average growth curves and g modified Kaplan–Meier curves of KPC mice from each of the indicated treatment groups. h Treatment protocol in NSG mice bearing AsPC-1 human PDAC tumors. i Average growth curves and j modified Kaplan–Meier curves of AsPC-1 human PDAC bearing NSG mice from each of the indicated treatment groups. k Representative multiplexed IF images (top panels) and quantification (bottom panels) of sections of end-point tumors from each of the indicated treatment groups stained for cleaved caspase-3, Ki-67, TGF-β or CD31 (red) as indicated and in each case co-stained with Pan-CK (green). Nuclei were stained with DAPI (blue). Scale bar, 100  μm. Data points are mean±SD (n = 5 per group) and groups were compared using one-way ANOVA analysis with Dunnett’s multiple comparison tests (b, c, f, i, k) or log-rank test (d, g, j). e The pancreas and ultrasound are created with BioRender.com. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. c MigR+MigR/Meso-CAR+MigR vs. FAP-CAR + FAP-CAR, p = 0.005 and 0.022, respectively. d MigR+MigR/Meso-CAR+MigR/Meso-CAR+Meso-CAR/FAP-CAR+MigR/FAP-CAR + FAP-CAR vs. FAP-CAR+Meso-CAR, p = 0.003, 0.002, 0.002, 0.002 and 0.002, respectively. g MigR+Meso-CAR/Meso-CAR+Meso-CAR/FAP-CAR + FAP-CAR vs. FAP-CAR+Meso-CAR, p = 0.003, 0.002 and 0.002, respectively. j p = 0.002 for all comparisons. k Ki-67: MigR+MigR/Meso-CAR+MigR/Meso-CAR+Meso-CAR vs. FAP-CAR + FAP-CAR, p = 0.008, 0.004 and 0.032, respectively. TGF-β: Meso-CAR+MigR/Meso-CAR+Meso-CAR vs. FAP-CAR+Meso-CAR, p = 0.008 and 0.002, respectively. Meso-CAR+MigR/Meso-CAR+Meso-CAR vs. FAP-CAR + FAP-CAR, p = 0.005 and 0.001, respectively. The p values for remaining comparisons are all <0.001 or <0.0001. Source data are provided as a Source Data file.

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