Fig. 2: The yield and stability of single-component immunogens are higher than those of AMA1 DI-DII alone and the AMA1 DI-DII-RON2L complex.
From: Structure-based design of a strain transcending AMA1-RON2L malaria vaccine
a All three immunogens were expressed at higher levels than AMA1 DI-DII and eluted as monomers by size exclusion chromatography (SEC). The inset in (a) confirms the high purity of the immunogens through reducing SDS-polyacrylamide gel electrophoresis (PAGE). b Purification yield from three separate purifications. Bars represent the mean yield from three separate purifications. c Differential scanning fluorimetry indicated that three immunogens have higher thermostability than AMA1 DI-DII and the AMA1 DI-DII-RON2L complex. d Tm from five independent measurements. Bars represent the mean. Source data are provided as a Source data file.
