Fig. 6: Impact of PVP01_0000100 binding on reticulocyte membrane properties.
a Membrane tension was measured using flickering spectroscopy for CD71+ reticulocytes in the absence of additional protein (untreated), and incubated with 100 μg/ml proteins (Pfs25 and PVP01_0000100 CTD). Number of cells per sample: untreated n = 65, Pfs25 n = 73, and PVP01_0000100 n = 79. Pairwise comparisons between groups used Tukey’s honest significant difference (HSD) test and Bonferroni correction. P** = 0.0015 (Reticulocytes Untreated vs PVP01_0000100 CTD), P** = 1.7 × 10−6 (Pfs25 vs PVP01_0000100 CTD). The fluctuations with contour detection of untreated red blood cell and in presence of proteins are shown in Supplementray Movies 1,2 and 3. b Wrapping energy was calculated using MATLAB. Data points for untreated are 57, for Pfs25 are 73 and for PVP01_0000100 sample are 77. Statistical comparison across groups was performed by one-way ANOVA with Bonferroni correction for multiple comparisons, while pairwise comparisons between groups used the Tukey HSD test. P** = 5.2 × 10−4 (Reticulocytes Untreated vs PVP01_0000100 CTD), P** = 1.3551 × 10−5 (Pfs25 vs PVP01_0000100 CTD). Each box, the central mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The whiskers extend to the most extreme data points not considered outliers, and the outliers are plotted individually using the ‘+‘ marker symbol. Source data is provided in the source data file.
