Fig. 1: The gene editing for B2M and workflow analysis. | Nature Communications

Fig. 1: The gene editing for B2M and workflow analysis.

From: Whole genomic analysis reveals atypical non-homologous off-target large structural variants induced by CRISPR-Cas9-mediated genome editing

Fig. 1

a Schematic presentation of human B2M gene and sequence of B2M exon 1 showing PAM site and the genomic target for sgRNA. Boxes represent exons of the B2M gene. b The sequences and chromatogram of the frameshift mutations in B2M-/- clones. The B2M-/-−1, −2, and −3 single-cell clones were isolated after CRISPR-Cas9-mediated genome editing. The DNA mutations of each allele were demonstrated by sequencing analysis. c Knockout of B2M in iPSCs abrogates surface expression of HLA class I (HLA-ABC) detected via flow cytometry. The red color represents the expression of HLA-ABC, and the gray color represents the isotype control. n = 4 replicates. The gating strategies are provided as a Source Data file.

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