Fig. 1: Loss of Pngl is associated with the upregulation of immune response-related genes. | Nature Communications

Fig. 1: Loss of Pngl is associated with the upregulation of immune response-related genes.

From: Gut barrier defects, intestinal immune hyperactivation and enhanced lipid catabolism drive lethality in NGLY1-deficient Drosophila

Fig. 1

a Principal component analysis (PCA) plot showing the variance among the genotypes used in RNA-seq. b Venn diagrams showing the overlap of differentially regulated genes in Pngl–/– with comparison to control (y w), Pngl+/– and Pngl–/–, Pngl Dp/+ in RNA-seq analysis. Number of both upregulated and downregulated genes shown in the Venn diagram were based on >1.5 fold-change. c Graph presents DAVID functional GO analysis of biological processes (BP) of downregulated (top), and upregulated (bottom) genes based on their –log10 of FDR. Numbers in parenthesis show the number of genes differentially expressed in each category and the numbers next to bars show the fold enrichment. d Graph showing expression of immune response-related genes in the indicated genotypes. Values are expressed as fold changes relative to control (y w). Mean ± standard deviation of three independent replicates is shown. Numbers on the bars indicate the P values. Significance is ascribed as P < 0.05 using one-way ANOVA with multiple comparisons followed by Šidák correction. Source data are provided as Source Data file.

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