Fig. 5: Gut microbiota does not explain gut barrier defects and lethality in Pngl mutants. | Nature Communications

Fig. 5: Gut microbiota does not explain gut barrier defects and lethality in Pngl mutants.

From: Gut barrier defects, intestinal immune hyperactivation and enhanced lipid catabolism drive lethality in NGLY1-deficient Drosophila

Fig. 5

a Graph showing quantification of gut barrier defect phenotype in germ-free control and Pngl–/– larvae (60 animals for each group from four independent experiments). b Graph showing the percentage of germ-free and conventionally reared control and Pngl–/– animals that reached the pupal stage (120 animals for each group from four independent experiments). c Graph showing % lethality rescue in germ-free and conventionally reared Pngl–/– and Pngl–/–; foxo+/– animals (n = 4 independent replicates). d Graph showing immune gene expression in germ-free control and Pngl–/– larvae (n = 3 independent replicates). In all panels, each circle represents an independent replicate, and mean ± standard deviation is shown. P values are indicated on bars. Significance is ascribed as P < 0.05 using two-way ANOVA followed by Šidák correction (b, c) or two-tailed unpaired student’s t-test (a, d). Source data are provided as Source Data file.

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