Fig. 6: Gut barrier defects induce starvation and Foxo-dependent induction of innate immune genes and lethality. | Nature Communications

Fig. 6: Gut barrier defects induce starvation and Foxo-dependent induction of innate immune genes and lethality.

From: Gut barrier defects, intestinal immune hyperactivation and enhanced lipid catabolism drive lethality in NGLY1-deficient Drosophila

Fig. 6

a Confocal images showing FITC-labeled dextan (yellow) in midguts from control and Poly D-fed larvae of indicated genotypes and quantification of gut barrier defect in these animals (n = 3 independent replicates). Scale bar is 250 µm. b Graph showing % adult eclosion in Poly D-fed control and foxo+/– animals (n = 4 independent replicates). c Graph showing immune response gene expression in control and Poly D-fed y w and foxo+/– midguts (n = 3 independent replicates). d Graph showing gene expression of lipases in control and Poly D-fed midguts of y w larvae (n = 3 independent replicates). e Western blot images and quantification graph showing Foxo and pFoxo levels in the midguts of Poly D-fed larvae of indicated genotypes (n = 3 independent replicates). f Confocal images showing DAPI (blue) and pJNK (red) staining and quantification in control and Poly D-fed y w midguts. The scale bar is 50 µm. n = 69 nuclei from four animals for each group. g Confocal images showing DAPI (blue) and pJNK (red) staining and quantification in control and Pngl–/– midguts. The scale bar is 50 µm. n = 56 (y w) and 66 (Pngl–/–) nuclei from four animals for each group. h Graph showing % lethality rescue upon enterocyte-specific knockdown of Drosophila JNK (bsk) in Pngl mutants (n = 3 independent replicates). i Graph showing innate immune gene expression in the indicated genotypes (n = 3 independent replicates). j Confocal images showing DAPI (blue) and Foxo (red) staining and quantification of Foxo nuclear localization in the indicated genotypes. The scale bar is 50 µm. n = 35–36 nuclei from three animals for each group. Each circle represents an independent replicate in (a–e, h, i) or a nucleus in (f, g, j). Mean ± standard deviation is shown in all graphs. P values are indicated on bars. Significance is ascribed as P < 0.05 using one-way ANOVA followed by Šidák correction (e, I, j), two-way ANOVA followed by Šidák correction (c) or two-tailed unpaired student’s t-test (a, b, d, f–h). Source data are provided as Source Data file.

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