Fig. 3: SPHKAP-type I PKA localizes to stacked ER cisternae at ER-PM junctions.
From: Neuronal ER-plasma membrane junctions couple excitation to Ca2+-activated PKA signaling
a EM images of SPHKAP-immunoperoxidase DAB reaction product acquired from the soma of a CA1 pyramidal neuron in a rat brain section. High-magnification images of insets are provided on right. Scale bars, 1 µm (left panel) and 500 nm (insets i & ii). Images are representative of results obtained from n = 2 rats. b EM image of SPHKAP-immunogold particles acquired from the soma of a CA1 pyramidal neuron in a mouse brain section. Scale bar, 200 nm. Images are representative of results obtained from n = 2 mice. c EM image of SPHKAP-immunogold particles and RI-immunoperoxidase reaction product acquired from the soma of a CA1 pyramidal neuron in a mouse brain section. Scale bar, 200 nm. Images are representative of results obtained from n = 2 mice.
