Fig. 7: SPHKAP promotes depolarization-induced transcription factor activation.
From: Neuronal ER-plasma membrane junctions couple excitation to Ca2+-activated PKA signaling
a Experimental paradigm to assess depolarization-induced transcription factor activation in cultured hippocampal neurons. b Images of phosphorylated CREB (pCREB) and c-Fos immunolabeling in control (4.7 mM K+ solution: 4.7 K) and depolarized (45 mM K+ solution: 45 K) neurons expressing scr or SPHKAP shRNA B. Scale bar, 10 µm. c Mean ± s.e.m pCREB and c-Fos immunofluorescence signal intensity; each point represents 1 cell (n = 10 [scr, 4.7 K pCREB], 12 [SPHKAP, 4.7 K pCREB], 15 [scr, 45 K pCREB], 14 [SPHKAP, 45 K pCREB], 25 [scr, 4.7 K c-Fos], 31 [SPHKAP, 4.7 K c-Fos], 20 [scr, 45 K c-Fos], and 30 [SPHKAP, 45 K c-Fos] cells. Statistical significance determined using one-way ANOVA followed by Tukey’s multiple comparisons test; *P = 0.0185, **P = 0.0051, ***P = 0.008 (pCREB) or 0.006 (c-Fos), ****P < 0.0001. Source data are provided as a Source Data file.
