Fig. 2: DNAJA2 regulates the stability of key centriolar satellite proteins.

a Representative images showing colocalization between DNAJA2 and centrosome proteins NUDC and PLK1 in both interphase (top) and M-phase (bottom) HeLa cells. b Confocal images showing colocalization between DNAJA2 and HSC70 at centrosomes of M-phase HeLa cells. Data in (a) and (b) were representative results from three independent experiments with similar outcomes. c Western blots showing protein levels of PCM1 and CEP290 in WT and DJ2^−/− 4T1 cells. d Quantifications of relative PCM1 and CEP290 levels (means ± SEM, n = 4 experimental repeats) in WT and DJ2^−/− 4T1 cells, as shown in (c). e Confocal images showing PCM1 and CEP290 signals (indicated by yellow arrows) in control and DJ2^−/− 4T1 cells. f Quantification (means ± SEM) of PCM1 and CEP290 intensities in control (n = 30 cells) and DJ2^−/− (n = 38 cells) 4T1 cells, as shown in (e). g Western blots showing protein stability of PCM1 and CEP290 in WT and DJ2^−/− 4T1 cells. Cells were treated with 50 μg/ml cycloheximide (CHX) for the indicated times and harvested for Western blotting analysis. h, i Quantifications (means ± SEM) of relative protein level of PCM1 (n = 4 experimental repeats) and CEP290 (n = 3 experimental repeats) as shown in (g). The relative protein levels were normalized to the level of the time 0 point in both groups. Scale bar, 10 μm. The P value in figure (i) was determined by two-tailed unpaired t test and the others were determined by two-tailed unpaired t test with Welch’s correction. Source data are provided as a Source data file.