Fig. 5: Elevated PCM1 level contributes to the mitotic defects in DNAJA2-deficient cells. | Nature Communications

Fig. 5: Elevated PCM1 level contributes to the mitotic defects in DNAJA2-deficient cells.

From: DNAJA2 deficiency activates cGAS-STING pathway via the induction of aberrant mitosis and chromosome instability

Fig. 5

a Western blots showing PCM1 expression levels in WT, DJ2−/− and DJ2−/− with control shRNA (DJ2−/−+shCtrl) or shRNA targeting PCM1 (DJ2−/−+shPCM1) HeLa cells. b, c Representative images and quantification (means ± SEM, n = 4 experimental repeats) of cells with micronuclei in DJ2−/−+shCtrl and DJ2−/−+shPCM1 HeLa cells. Micronucleus were indicated by yellow arrows. d Representative images showing spindle morphologies (green) of DJ2−/−+shCtrl and DJ2−/−+shPCM1 HeLa cells. e Quantification (means ± SEM, n = 3 experimental repeats) of cells with bipolar and abnormal spindles in DJ2−/−+shCtrl and DJ2−/−+shPCM1 HeLa cells. All multipolar, monopolar, and diffuse-polar spindles were counted as abnormal spindles. f, g Representative images and quantification (means ± SEM) of cells with micronuclei in HeLa cells expressing empty vector (n = 4 experimental repeats), WT (n = 3 experimental repeats), and KFERQ mutant (2AA) (n = 3 experimental repeats) HA-PCM1. Scale bar, 20 μm. P values were determined by two-tailed unpaired t test with Welch’s correction. Source data are provided as a Source data file.

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