Fig. 2: Lrig1 is required for the suppressive population of T_reg and Th17 cells.

a Suppressive potential of CD4⁺Lrig1⁺ cells, CD4⁺Lrig1⁻ cells, or CD4⁺GFP⁺ cells (Foxp3⁺) toward eFlour 670-labeled effector T cells with a different ratio. Effector only or CD4⁺Foxp3⁺ T cells: n = 4, CD4⁺Lrig1⁺ T cells or CD4⁺Lrig1⁻ T cells: n = 5. Data are expressed as mean ± S.E.M (in ratio 4:1 *P = 0.02, **P = 0.007; in ratio 1:1 *P = 0.01, **P = 0.002, ***P < 0.001). b The level of IL-10 in the culture medium of total splenocytes, activated CD4⁺ T cells, CD4⁺Foxp3⁺ T cells, or CD4⁺Lrig1⁺ or Lrig1⁻ T cells in mouse spleen (n = 3). Data are expressed as mean ± S.E.M (*P = 0.03, ***P < 0.001). c Representative images (left) and quantification (right) of suppression activity of CD4⁺Foxp3⁺Lrig1^hi or CD4⁺Foxp3⁺Lrig1^low T cells expressing a similar level of Foxp3 (n = 6). Data are expressed as mean ± S.E.M (***P < 0.001). d, e Comparison of the suppressive activity of Lrig1⁺ and Lrig1⁻ cells from mouse Th17, iT_reg, Th0, or Th1 cells (d) (n = 3) or mouse Lrig1⁺ and Lrig1⁻ cells from CD25⁻CCR6⁺ T cells or CD25⁺ T cells in the splenocytes (e) (n = 3). Data are expressed as mean  ±  S.E.M (in d 2:1 ratio, ***P < 0.0001 Lrig1⁻ Th17 vs Lrig1⁺ Th17, ***P = 0.0003 Lrig1⁻ iT_reg vs Lrig1⁺ iT_reg; 1:1 ratio, *P = 0.012, ***P = 0.0007; 1:2 ratio, *P = 0.0127, **P = 0.0099; in e ***P < 0.0001). f The suppressive potential of human LRIG1⁺ and LRIG1⁻ cells from human blood differentiated Th17 or iT_reg cells (n = 3). Data are expressed as mean ± S.E.M (*P = 0.0479 LRIG1⁻ Th17 vs LRIG1⁺ Th17, *P = 0.0471 LRIG1⁻ iT_reg vs LRIG1⁺ iT_reg, **P = 0.0014 LRIG1⁻ Th17 vs LRIG1⁺ Th17, **P = 0.0032 LRIG1⁻ iT_reg vs LRIG1⁺ iT_reg). Ordinary one-way ANOVA with Dunnett’s multiple comparisons test (b) or two-way ANOVA with Tukey’s multiple comparisons test (a, c–f). All experiments were repeated at least three times. Source data are provided as a Source Data file.