Fig. 2: Sulfur-based energy metabolism of Taurinivorans muris LT0009.
From: Ecophysiology and interactions of a taurine-respiring bacterium in the mouse gut
a Cell cartoon of the central sulfur and energy metabolism of LT0009 as determined by genome, transcriptome, and proteome analyses. Genes/proteins detected in the transcriptome and proteome of LT0009 grown with taurine, sulfolactate, or thiosulfate as electron acceptor are shown by colored circles and squares, respectively. Circle size indicates gene transcription level normalized as TPM. Proteins of all transcribed genes were also detected in the proteome, with the exception of AprAB, TauA (TAU_v1_0027, TAU_v1_1344), TauB, TauC, TauE, DctMQ2, SlcG, DsrEFH, AscA, two [FeFe] hydrogenases (TAU_v1_1126, TAU_v1_1901), cytochrome c and Sdh. Protein complexes (e.g., Rnf, SlcFGH, DctPMQ2, Atp) are not shown with transcriptome and proteome data because at least one gene/protein of the complex units was not detected. The gene annotations are listed in Supplementary Data 7. b Anaerobic growth tests of strain LT0009 with various substrates (n = 3 biologically independent cultures). Electron donors: All substrates were added at 10 mmol/l concentration, except acetate (20 mmol/l), which was added as carbon source together with H2. Electron acceptors: The different sulfur compounds were added at 10 mmol/l concentration together with pyruvate, lactate, and 1,4-naphthoquinone. OD600: optical density at 600 nm. c Organization of sulfur metabolism genes in the LT0009 genome. Numbers show the RefSeq locus tag with the prefix TAUVO_v1. d Comparative transcriptome and proteome analysis of LT0009 grown with lactate and taurine, sulfolactate, or thiosulfate as electron acceptor. Numbers following protein names refer to RefSeq locus tag numbers (prefix TAUVO_v1). Protein expression was normalized to DsrC for each growth condition. Bars represent averages and error bars represent one standard deviation (n = 3 biologically independent cultures). Asterisk indicates significant (p < 0.05, exact p values are provided in Supplementary Data 1) differences in gene transcription/protein expression compared to growth with taurine (5% false discovery rate, DESeq2 Wald test). TCA, tricarboxylic acid cycle; WL, Wood-Ljungdahl pathway; PEP, phosphoenolpyruvate; DHPS, 2,3-dihydroxypropane-1-sulfonate; TPM, transcripts per million. Source data are provided as a Source Data file.
