Fig. 1: Coating liposome with DNA tiles for protein display.
From: Functionalization and higher-order organization of liposomes with DNA nanostructures
Preformed liposomes are first modified with ssDNA (i, green line) by cholesterol-membrane association, then coated with 6HB DNA tiles (ii) by DNA hybridization (iii). An additional ssDNA handle on each tile (ii, red line) is reserved for further functionalization with proteins, such as streptavidin (SA) by biotin-SA interaction (iv), or v-SNARE by DNA-tethered nanodisc (DNDv) reconstitution (v). Both liposomes and NDs contain 65% DOPC, 15% DOPE, and 20% DOPS. Representative negative-stain TEM images are shown beside the cartoon model of each construct. Scale bars: 50 nm.
