Fig. 1: Experimental roadmap of the study. | Nature Communications

Fig. 1: Experimental roadmap of the study.

From: DNA methylation profiling to determine the primary sites of metastatic cancers using formalin-fixed paraffin-embedded tissues

Fig. 1

a Flow chart of participants. b Schematic diagram of the FFPE-RRBS protocol. Genomic DNA was isolated from FFPE tissue. The degraded genomic DNA was then end-polished by dephosphorylation. Subsequent enzymatic reactions, including MspI digestion, end-repair, A-tailing, and adapter ligation, were performed in the same tube without DNA cleanup. After bisulfite conversion, the library DNA was enriched by PCR and sequenced on an Illumina NovaSeq 6000 sequencer.

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