Fig. 1: Gene discovery at the Rph7 locus in Cebada Capa.

a Genetic mapping narrowed the Rph7 locus between flanking markers within genes HvGAD1 and HvPG4. b Schematical overview of previous sequenced BAC contigs²⁹ (grey). RNA-Seq data revealed the gene expression of previous reported candidate genes (blue) and identified a further four expressed candidate genes labelled in purple. Among the four identified expressed candidates, three genes are upregulated after infection (highlighted in red dashed box) in the resistant lines Cebada Capa and the near isogenic line BW758 (Bowman+Rph7) compared to susceptible Bowman at day 6. c Expression profile of ZnF-BED1 (Rph7). Samples labelled with white background were treated as mock control, while pink background labelled shows the expression patterns are from barley leaves infected with Rph7-avirulent Puccinia hordei pathotype 5457 P+ for Bowman, BW758 and Cebada Capa. The data presented in all cases captures biological replication (n = 3) where the box encompasses two middle quartiles, with central line showing the mean. Whiskers extend to the furthest data point within 1.5 times the interquartile range. d Complementation results showing the same phenotypic responses between the T₁ transgenic Golden Promise + Rph7 line B114-1 and the Rph7-carrying positive control BW758 at the seedling stage 10 days after infection with Puccinia hordei pathotypes 5477 P− and 200 P+ that elicit intermediate and low infection types respectively. Leaf rust susceptible controls Golden Promise and Bowman were also inoculated with 5477 P− and demonstrate the Rph7 specificity. Source data are provided as a Source Data file.