Fig. 3: Chimeric eccDNA containing a truncated EVD-gene fusion corresponds to a chimeric genomic insertion in A. thaliana ddm1 rdr6 polIV mutant. | Nature Communications

Fig. 3: Chimeric eccDNA containing a truncated EVD-gene fusion corresponds to a chimeric genomic insertion in A. thaliana ddm1 rdr6 polIV mutant.

From: Extrachromosomal circular DNA and structural variants highlight genome instability in Arabidopsis epigenetic mutants

Fig. 3

a Dot plot between an ONT read from the ddm1 rdr6 polIV mutant mobilome-seq and corresponding EVD (color code as in Fig.2) and AT5G66440 sequence (red). The deduced chimeric circle is depicted on the left, consisting of a truncated EVD fragment (701 bp) fused to a truncated AT5G66440 gene fragment (708 bp). b eccDNA-seq ONT reads containing EVD were selected and mapped to the reference genome and shown at the AT5G66440 locus. c New TE insertions detected in the ddm1 rdr6 polIV mutant genome. A new copy of EVD is detected at the AT5G66440 locus (red triangle). d The EVD insertion at AT5G66440 created a 5 bp target site duplication (TSD, black line), visible thanks to read mapping locations ending or starting at the same position on the reference.

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