Fig. 3: Interception synthetic memory with expanded information processing and operator variation.

a A schematic summarizing the mechanism and genetic construct (deletion circuit) used to assess A118 recombinase interception with variable repressors directed at different operators placed in the P + 1 position (described in Fig. 2j). The A118 recombinase and relevant repressor are constitutively expressed in all cases. The repressors used are comprised of two modular domains: (1) a regulatory core domain that allows the repressor to be induced by a different ligand, and (2) a DNA-binding domain that allows the repressor to bind to a different operator. In STATE 1, repressor binding at the operator blocks recombinase function, protecting the circuit from deletion. Inducing the repressor brings the circuit to STATE 2, where the recombinase can access the attP site to recombine the circuit (bringing the circuit to STATE 3). b Assay data for intercepted (minus ligand) circuits vs. deprotected (induced) circuits using the repressor E⁺ across six different DNA-binding domain/operator pairs. Assay data using the same set of DNA-binding domain/operator pairs as (b) paired with different regulatory core domains as follows: c R⁺, d F⁺, e G⁺, and f I⁺. (inset) Symbols for the circuit parts and modular repressor components used, including six DNA-binding domains conferring alternate DNA recognition, the six DNA operators where those DNA-binding domains can bind (color-matched), and the five regulatory core domains sensitive to different ligands. Source data are provided as a Source Data file. Data in (b–f) represent the average of n = 6 biological replicates. Error bars correspond to the SEM of these measurements.