Fig. 4: Characterization of P. occidentalis promoters and terminators. | Nature Communications

Fig. 4: Characterization of P. occidentalis promoters and terminators.

From: Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid

Fig. 4

a OD-normalized fluorescence intensity of P. occidentalis Y-7552 strains expressing common GFP gene variants. GFP-encoding variants were expressed using PTDH3 and TRPL3 from P. occidentalis Y-7552. GFP-expressing colonies are shown with UV exposure. b OD-normalized fluorescence intensity of mNeonGreen expressed from 12 P. occidentalis gene promoters with the RPL3 terminator. c OD-normalized fluorescence intensity of mNeonGreen expressed from the GPM1 promoter and 12 P. occidentalis gene terminators. Error bars represent the mean ± s.d. of n = 3 independent biological samples. All GFP constructs were integrated into the FCY1 locus of P. occidentalis. Source data are provided as a Source Data file.

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