Table 4 Pichia target sites utilized in this study

From: Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid

Organism

Target site ID

Target site sequencea

Reference

P. kluyveri

ADE2

TAGAGAAAATGTCTGATTAATGG

This study

P. occidentalis

ADE2

TTAATGAACCCGGTTGTTGATGG

This study

 

FCY1

TAAGTTTCTGTTGTCGCCCTGGG

This study

 

ARO3

ATCAAGGGCAAACTTCCTTGTGG

This study

 

ARO4

TGACATCTTGTGAATCTGTGTGG

This study

 

01b

TTTTGGTAGCGTGCCTTGTACGG

This study

 

03a

TGACTATACACTCTGTCTACAGG

This study

 

03b

TTTGTGTAAAACTATTAAGCGGG

This study

Other

G418R

TCCGTACTCCTGATGATGCATGG

This study

 

HygR

TCTCGATGAGCTGATGCTTTGGG

This study

  1. aPAMs are underlined.