Table 5 Expression cassettes utilized in this study

eGFP

P_TDH3-eGFP-T_RPL3

FCY1

SuperfolderGFP (sfGFP)

P_TDH3-sfGFP-T_RPL3

FCY1

Envy GFP

P_TDH3-Envy-T_RPL3

FCY1

mNeonGreen GFP

P_TDH3-mNeonGreen-T_RPL3

FCY1

P_ASC1-mNeonGreen

P_ASC1-mNeonGreen-T_RPL3

FCY1

P_CYC1-mNeonGreen

P_CYC1-mNeonGreen-T_RPL3

FCY1

P_FBA1-mNeonGreen

P_FBA1-mNeonGreen-T_RPL3

FCY1

P_GPM1-mNeonGreen

P_GPM1-mNeonGreen-T_RPL3

FCY1

P_HHF1-mNeonGreen

P_HHF1-mNeonGreen-T_RPL3

FCY1

P_HHF2-mNeonGreen

P_HHF2-mNeonGreen-T_RPL3

FCY1

P_HTB2-mNeonGreen

P_HTB2-mNeonGreen-T_RPL3

FCY1

P_PGK1-mNeonGreen

P_PGK1-mNeonGreen-T_RPL3

FCY1

P_RNR2-mNeonGreen

P_RNR2-mNeonGreen-T_RPL3

FCY1

P_TEF1-mNeonGreen

P_TEF1-mNeonGreen-T_RPL3

FCY1

P_TPI1-mNeonGreen

P_TPI1-mNeonGreen-T_RPL3

FCY1

mNeonGreen-T_ASC1

P_GPM1-mNeonGreen-T_ASC1

FCY1

mNeonGreen-T_CYC1

P_GPM1-mNeonGreen-T_CYC1

FCY1

mNeonGreen-T_EFM1

P_GPM1-mNeonGreen-T_EFM1

FCY1

mNeonGreen-T_ENO2

P_GPM1-mNeonGreen-T_ENO2

FCY1

mNeonGreen-T_HTB2

P_GPM1-mNeonGreen-T_HTB2

FCY1

mNeonGreen-T_IDP1

P_GPM1-mNeonGreen-T_IDP1

FCY1

mNeonGreen-T_PGK1

P_GPM1-mNeonGreen-T_PGK1

FCY1

mNeonGreen-T_RNR2

P_GPM1-mNeonGreen-T_RNR2

FCY1

mNeonGreen-T_SRP54

P_GPM1-mNeonGreen-T_SRP54

FCY1

mNeonGreen-T_TPI1

P_GPM1-mNeonGreen-T_TPI1

FCY1

mNeonGreen-T_VMA2

P_GPM1-mNeonGreen-T_VMA2

FCY1

PaAROZ

P_TDH3-PaAROZ-T_RPL3

ARO4

PoARO4^K225L

P_TDH3-PoARO4^K225L-T_EFM1

01b

Ec.aroB-Ec.aroD

P_TDH3-Ec.aroB-T_IDP1-LTP1-P_GPM1-Ec.aroD-T_VMA2

ARO3

Kp.aroY-ScPAD1

P_TDH3-Kp.aroY-T_SRP54-LTP1-P_TEF1-ScPAD1-T_RPL15A

03b

CaHQD2-Kp.aroY

P_TDH3-CaHQD2-T_SRP54-LTP1-P_TEF1- Kp.aroY-T_RPL15A

03a