Fig. 7: Cecal microbiota composition in recipient mice after microbiota transfer.

Mice were inoculated with cecal microbiota from mice fed MF or A diet and subsequently fed MF diet for 9 weeks. a Quantification of total bacterial counts in cecum by qPCR. b Number of observed species of inoculums and in recipient mice fed MF microbiota and inoculated with either MF or A diet microbiota. Principal coordinates analysis (PCoA) based on c Bray–Curtis dissimilarity and d unweighted UniFrac distance (means of ranked distances between recipients of microbiota from mice fed diets A or MF and of the inoculums used to colonize the mice). e Differential abundance of bacterial taxa at phylum and genus levels for mice after inoculation with cecal microbiota from mice on MF or A diets. Two-sided Wilcoxon rank sum test FDR-adjusted p value < 0.1. Direction and coloring of triangles indicate Cliff’s Delta effect size (positive effect size and blue color indicate increased relative abundance in diet A microbiota recipients). The triangle size and left marginal bar plot depict log transformed abundance of individual taxa. The right marginal bar plot shows proportion of samples containing respective taxa. n = 9 (MF microbiota inoculum), 15 (MF microbiota recipients), 8 (A microbiota inoculum), 14 (A microbiota recipients). Significant p values determined by two-sided Mann–Whitney U-test are displayed in the figure. Data are presented as mean ± SEM. See also Supplementary Fig. 10. Source data are provided as a Source data file.