Fig. 1: Larval fat body is resistant to cell death by several cellular stresses.

a Simplified schematic of the apoptotic signaling cascades induced by DNA and ER stresses. b, c Induction of cell death in the fat body by pro-apoptotic genes. Mid-third instar larvae grown at 18 °C were incubated at 29 °C for 24 h for gene induction. Fat bodies were dissected and stained for cDcp1, F-actin, and DNA (b). Quantification of nuclear diameter in cDcp1-negative (–) and positive (+) fat body cells in each genotype (c). Horizontal lines indicate the means of individual groups. Values of n indicate the number of nuclei from multiple animals. Unpaired two-sided Mann–Whitney U-test. d Cg-Gal4 activity in the larval fat body by using the dual-color fluorescent reporter, TransTimer. The experimental condition is identical to those described in (b). Fat bodies were dissected and stained for GFP and DNA. e Evaluation of the cell death phenotype in the fat body. Fat bodies of the indicated genotypes were dissected from the mid-third instar larvae 24 h after the temperature shift and stained for cDcp1, F-actin, and DNA. f Apoptosis in wing discs. Wing discs of the indicated genotypes were dissected from the late-third instar larvae and stained for cDcp1, GFP, and DNA. nub-Gal4 was used to drive transgene expression in the wing pouch. Scale bars: 100 μm (b, d–f).