Fig. 6: Loss of Nacα induces ER stress and cell death through JNK signaling.

a, b ER stress as determined by the expression of Xbp1-GFP. Fat bodies of the indicated genotypes were dissected from the early third instar and stained for GFP and DNA (a). Quantification of the mean GFP fluorescence intensity per nucleus (b). c Relative gene expression levels in fat bodies as determined by qRT-PCR. Fat bodies of the indicated genotypes were dissected from the mid-third instar (M3rd) and subjected to the experiments. d, e Cell death in the fat body. Fat bodies of the indicated genotypes were dissected from the late third instar (L3rd) and stained for cDcp1, F-actin, and DNA (d). Quantification of the nuclear diameter of fat body cells (e). f Apoptosis in wing discs. Wing discs of the indicated genotypes were dissected from the L3rd and stained for cDcp1 and DNA. The quantification of cDcp1 signals is shown in Supplementary Fig. 5d. g Adult wing morphology of the indicated genotypes. Representative images of male flies are shown. h Relative gene expression levels in Nacα knockdown fat body. Fat bodies were dissected from the M3rd and subjected to qRT-PCR analysis. Horizontal lines indicate the means of individual groups (b, e). Values of n indicate the number of cells (b) or nuclei (e) from multiple animals. For appropriate panels, results are presented as the mean ± SD, n = 4 batches (c, h); unpaired two-sided Mann–Whitney U-test (b), one-way ANOVA with Dunnett’s post hoc test (c), Kruskal–Wallis test followed by Dunn’s post hoc test (e), unpaired two-tailed Student’s t-test (h). Scale bars, 50 μm (a), 100 μm (d, f).