Fig. 5: Functional separation of BCC-related CAFs differs from cSCC.

a Violin plot showing the average gene expression of cSCC-related CAF marker genes in fibroblasts that were detected in the integrated analysis of nine BCC samples from Yerly et al. ⁴⁶, and Guerrero-Juarez et al. ⁴⁵. All indicated comparisons: p value < 2.2e-16. b Violin plot showing the average gene expression of the known CAF marker genes FAP and ACTA2 in fibroblasts from all BCC samples. All indicated comparisons: p value < 2.2e-16. c UMAP plot visualizing four actual CAF clusters. d Violin plots depicting the average gene expression of cSCC-related iCAF (CXCL12, JUND, TSC22D3) and myCAF (MMP11, TAGLN, COL11A1) marker genes in the detected CAF clusters from BCC. CXCL12 CAF 0 vs. 2: p value < 2.2e-16, CXCL12 CAF 1 vs. 2: p value = 2.78e-06, CXCL12 CAF 2 vs. 3: p value < 2.2e-16, JUND CAF 0 vs. 2: p value = 8.37e-09, JUND CAF 1 vs. 2: p value = 0.0002782, JUND CAF 2 vs. 3: p value = 0.0115, TSC22D3 CAF 0 vs. 2: p value < 2.2e-16, TSC22D3 CAF 1 vs. 2: p value = 4.40e-12, TSC22D3 CAF 2 vs. 3: p value = 1.00e-15, MMP11 CAF 0 vs. 2: p value < 2.2e-16, MMP11 CAF 1 vs. 2: p value < 2.2e-16, MMP11 CAF 2 vs. 3: p value < 2.2e-16, TAGLN CAF 0 vs. 2: p value < 2.2e-16, TAGLN CAF 1 vs. 2: p value < 2.2e-16, TAGLN CAF 2 vs. 3: p value < 2.2e-16, COL11A1 CAF 0 vs. 2: p value = 4.47e-15, COL11A1 CAF 1 vs. 2: p value = 5.57e-15, COL11A1 CAF 2 vs. 3: p value < 2.2e-16). For violin plots, statistical analyses were performed using a two-sided Wilcoxon Rank Sum test (*: p value < 0.05, ***: p value < 0.001, ****: p value < 0.0001). FB fibroblast, iCAF inflammatory cancer-associated fibroblast, myCAF myofibroblastic cancer-associated fibroblast, BCC basal cell carcinoma, cSCC cutaneous squamous cell carcinoma.