Fig. 2: Photo-activated LiSmore drives type I interferon production in BMDCs.

a Scheme for the experimental setup. Bone marrows from C57BL/6J mice were cultured in GM-CSF to induce BMDCs. On days 7 and 8, BMDCs were transduced twice with Control (GFP-CRY2clust) or LiSmore (GFP-CRY2clust-pLxIS). After 36 h, GFP⁺ BMDCs were sorted and replanted in 48-well plates. BMDCs were either shielded from light (Dark) or exposed to blue light (Light; 470 nm, 1 mW/cm²; 20 s ON, 5 min OFF cycles). b ELISA analysis of secreted IFN-α and IFN-β concentrations in the supernatants collected from the four indicated groups with or without light stimulation for 18 h. n = 6 biological replicates (mean ± SD); Two-sided unpaired Student’s t-test. c Immunoblot analysis of TBK1, IRF3, and p65 phosphorylation in BMDCs in the absence (black bar) or presence of light stimulation (blue bar; 470 nm, 1 mW/cm²; 20 s ON, 5 min OFF cycles) for the indicated durations (0–8 h). Non-transduced BMDCs were treated with 2’3’-cGAMP (2 μg/ml) under the same timeframe side-by-side. Results are representative of two independent experiments.