Fig. 6: Photo-activated LiSmore suppresses distant tumor growth in a syngeneic bilateral B16-F10 melanoma model.
a Overview of the experiment design. 3 × 105 B16-F10 melanoma cells without OVA transduction were injected (s.c.) in the right and left flanks of WT CD45.1 B6 mice. Mice received intratumoral PBS, or cGAMP (10 μg/mouse) on the right side at day 7, 10.5, and 14 after tumor inoculation, or were transferred with BMDCs expressing Control or LiSmore (5 × 105 cells/mouse) at day 7. The right side of the tumor (the primary site) was subjected to pulsed blue light stimulation for 7 days (470 nm; 2 mW/cm2; 20 s ON + 5 min OFF; 6 h per day), while the left side (the distal site) was shielded from blue light (dark). b Left, schematic showing the treatment in the bilateral melanoma model. Right, individual growth curves of tumors in the primary (upper panels) and distant sites (lower panels) for the indicated treatment groups (n = 5 mice per group). c Primary and distal tumor growth in the indicated groups of mice (n = 5 mice per group; mean ± SD). Two-sided unpaired Student’s t-test.
