Fig. 1: Structural changes associated with P38A mutation.
a A CA hexamer is shown in surface view representation with three neighboring intra-hexamer CA monomers colored in orange (subunit ΄), yellow (subunit without prime symbol), and green (subunit ΄΄); the other three are shown in gray. P38A mutation site is marked with a red star in the yellow subunit. The P38A mutation affects regions in three subunits, shown in light blue surface view. b–d Superposition of WT (cartoon ribbons of three neighboring subunits colored in green, yellow, and orange) and P38A (in blue) CA. The mutation alters inter-hexamer CANTD-CANTD b, d and CANTD-CACTD interfaces c. Specific residues affected by P38A mutation (in red) are shown as sticks. For clarity, residues E35, V36, I37 are not shown. Dashed lines are shown between residues that are within 4 Å. Box colors in b–d correspond to the boxed regions in a. Dashed box in b is an insert of a region within the other box in b. For reference, the relative position of the revertant site, T216΄΄, not mutated here, is shown in red in c.
