Fig. 9: PGC-1α regulates the mRNA nuclear export of non-canonical PGC-1α target transcripts.

Sham, FLAG-tagged PGC-1α WT (WT-res) and FLAG-tagged PGC-1α ΔRS (ΔRS-res) cell lines were induced with doxycycline for 6 days. a, b RNA immunoprecipitation (RIP) assays of transcripts predicted to be novel mRNA nuclear export targets of PGC-1α. Cells were subjected to UV-C cross-link prior to anti-FLAG immunoprecipitation. Purified RNA was analysed by qRT-PCR and expressed as % of the input. RIPs were performed in three independent experiments (Mean ± SEM; two-way ANOVA with Turkey’s correction for multiple comparisons; **p < 0.01, ***p < 0.001, ****p < 0.0001; N = 3). c Total, nuclear and cytoplasmic levels of fractionated RNA transcripts were quantified in three independent experiments by qRT–PCR following normalization to U1 snRNA levels and to 100% in Sham cell line (Mean ± SEM; two-way ANOVA with Tukey’s correction for multiple comparisons, NS: not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; N = 3). d Western blots from Sham, WT-res and ΔRS-res cell lines induced with doxycycline for 6 days. Blots were probed for PGC-1α, FLAG, GLS, XRCC5, XRCC6, NHEJ1 and α-tubulin antibodies. e Western blots in (d) were quantified in three independent experiments (Mean ± SEM; one-way ANOVA with Tukey’s correction for multiple comparisons; *p < 0.05, **p < 0.01, ***p < 0.001; N = 3). For panels a–c and e, source data are provided with details of statistical tests and exact p-values as a Source data file.