Fig. 2: Mc5r expression in mouse ILC2s.

a–d Adoptive transfer of ILC2s into Rag2^−/−Il2rg^−/− female mice (6–8 weeks). Lung ILC2s (4 × 10⁵) from IL-33 treated mice were injected intravenously into Rag2^−/−Il2rg^−/− recipients, followed by UVB radiation (2.5 kJ/m²) before i.t. administrated with IL-33 (30 ng per mouse) for 3 days. Rag2^−/−Il2rg^−/− mice without ILC2s transfer were used as controls. a The experimental strategy of ILC2 transfer. b Abundance of eosinophils in BALF of recipients (n = 4, 4, 7, 7 from left to right). c The levels of IL-5 and IL-13 in the BALF were measured by ELISA (n = 7, 7 from left to right). d Absolute counts of lung eosinophils (n = 4, 4, 7, 7 from left to right); Percentage and number of ILC2s from recipients upon ILC2 transfer and UVB radiation (n = 7, 7 from left to right). e Hypothalamic–pituitary axis. f Scatter plot showing the FPKM for nerve-related gene expression in lung ILC2s sorted from naïve mice. Mc-r and opioid receptor gene expression in lung ILC2s sorted from naïve mice and analyzed by RNA-seq are shown as a heatmap. g Relative expression of Mc-r in sorted lung ILC2s (n = 3/group). h Relative expression of Mc5r in the indicated populations of sorted immune cells from the lungs (B cells: FVD⁻CD3⁻CD19⁺; CD4⁺ cells: FVD⁻CD3⁺CD4⁺; CD45⁺ cells: FVD⁻CD45⁺; CD8⁺cells: FVD⁻CD3⁻CD8⁺; DCs: FVD⁻CD45⁺CD103⁺MHC-II⁺CD11C⁺; Eosinophils: FVD⁻CD45⁺CD11c^−/loSiglecF⁺; AMs alveolar macrophage, FVD⁻CD45⁺Ly6G⁻CD11C⁺SiglecF⁺; interstitial macrophages IM: CD45⁺Ly6G⁻SiglecF⁻CD11c⁺CD11b⁺F4/80⁺; Neurophils: FVD⁻CD45⁺Ly6G⁺CD11B⁺; ILC2s: FVD⁻CD45.2⁺LIN⁻CD90.2⁺CD127⁺ST2⁺) (n = 3/group). Gene expression was analyzed by qRT-PCR and normalized with Hprt using the 2^−△△Ct method. Each symbol represents an individual mouse (b–d, g, h). The bars and error bars show the means ± SEMs. Data are representative of two or more independent experiments. For statistical analysis, the following tests were used. b–d Two-tailed unpaired Student’s t-test.